| Dr. Ying Fang |
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Assistant Professor Email: ying.fang@sdstate.edu Phone: 605-688-6647 Fax: 605-688-6003 Education: BS. Biology. P. R. China. MS. Entomology. Iowa State University. MS. Microbiology. South Dakota State University. PhD. Veterinary Microbiology. South Dakota State University. Specialty/Certification: Molecular Virology. Professional Emphasis: Dr. Ying Fang’s research laboratory has focused on the molecular characterization of porcine reproductive and respiratory syndrome virus (PRRSV), development and evaluation of PRRSV vaccines and diagnostic assays. The laboratory has recently successfully constructed a full-length cDNA infectious clone of European-like Type 1 PRRSV. Current efforts are directed towards the use of this reverse genetics system to study PRRSV pathogenesis and develop next generation of genetically engineered PRRSV vaccines. Dr. Ying Fang is a member of SDSU Hybridoma Facility. She is presently directing the establishment of a panel of monoclonal antibodies against PRRSV non-structural proteins. Collaborating project also involves in developing monoclonal antibodies against SARS-CoV nucleocapsid protein. Recent Professional News: Currently Funded Grants:- Fang, Y., D. Benfield, R. Rowland, E.A. Nelson, and J. Christopher-Hennings. Accurate ELISA test development for PRRSV serology: Evaluation of cysteine protease domains of nsp2 as potential diagnostic targets. National Pork Board. Fang, Y., M. Murtaugh and E.A. Nelson. Production of monoclonal antibodies against the PRRSV non-structural proteins. USDA NRI PRRSV Integrated. Fang, Y. Genetic marker development in the Nsp2 region of a European-like PRRSV: Implications for future recombinant marker vaccine development. USDA NRI Rowland, R.R.R. and Y. Fang. Preparation of monoclonal antibodies against the SARS virus N protein. NIH-Midwest Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research. Selected Publications:- Fang, Y., Pekosz, A., Haynes, L., Nelson, E., and Rowland, R. R. R. 2005. Production and characterization of monoclonal antibodies against the nucleocapsid protein of severe acute respiratory syndrome coronavirus (SARS-CoV), in: The Nidoviruses: The Control of SARS and Other Nidovirus Diseases. Springer, New York, In Press. W-H. Wu, Y. Fang, R. R. R. Rowland, Jane Christopher-Hennings, K-J. Yoon and E. A. Nelson. 2005. The 2b protein as a minor structural component of PRRSV. Virus Res. In Press. Rowland R. R. R., V. Chauhan, Y. Fang, A. Pekosz, M. Kerrigan, M. Burton. 2005. Intracellular localization of SARS coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in vero cells. J. Virol. In press. Fang, Y., D.-Y. Kim, S. Ropp, P. Steen, J. Christopher-Hennings, E.A. Nelson and R.R.R. Rowland. 2004. Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. Virus Res. 100(2): 229-235. Ropp, S.L., C.E. Mahlum-Wees, Y.Fang, E.A. Nelson, K.D. Rossow, M. Bien, B. Arndt, S. Preszler, P. Steen, J. Christopher-Hennings, J.E. Collins, D.A. Benfield and K.S. Faaberg. 2004. Characterization of emerging European-like PRRSV isolates in the United States. J. Virol. 78, 3684-3703. Wasilk, A., J. Callahan, J. Christopher-Hennings, B.T. Gay, Y. Fang, M. Dammen, M. Torremorell, D. Polson, M. Mellencamp, E.A. Nelson and W. Nelson. 2004. Detection of U.S. and Lelystad/European-like porcine reproductive and respiratory syndrome virus and relative quantitation in boar semen and serum by real-time PCR. J. Clin. Micro. 42: 4453-4461. Ferrin, N.H., Y. Fang, C.R. Johnson, M.P. Murtaugh, D.D. Polson, M. Torremorell, M. Gramer and E.A. Nelson. 2004. Validation of a Blocking ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Imm. 11:503-514. Rowland, R. R. R, P. Schneider, Y. Fang, S. Wootton, D. Yoo, and D. A. Benfield. 2003. Peptide domains involved in nuclear import, export and cytoplasmic retention of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. Virology. 316: 135-145. Christopher-Hennings, J., M.A. Dammen, S.R. Weeks, W.B. Epperson, S.N. Singh, G.L. Steinlicht, Y. Fang, J.L. Skaare, J.L. Larson, J.B. Payeur and E.A. Nelson. 2003. Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces. J. Vet. Diagn. Invest. 15:87-93. Fang, Y., W.-H. Wu, J.L. Pepper, J.L. Larsen, S.A.E. Marras, E.A. Nelson, W.B. Epperson and J. Christopher-Hennings. 2002. Comparison of real-time quantitative PCR using molecular beacons to nested PCR and culture methods for the detection of Mycobacterium avium (subspecies paratuberculosis) in bovine fecal samples. J. Clin. Micro. 40:287-291. Wu, W.-H., Y. Fang, R.R. Rowland, J. Christopher-Hennings and E.A. Nelson. 2001. A 10 kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virology 287:183-191. |
